Abstract: Biomass-bioenergy and biofuel have a great potential in alternative renewable energy production and carbon reduction. Biomass can be converted into a variety of bioenergy and biofuels via fermentation, pyrolysis, gasification or combustion with carbon capture and storage. In this paper, we evaluate the techno-economic feasibility and the environmental impacts of those conversion technologies with major terrestrial and aquatic biomass feedstocks and develop a mix-integer linear programming model to incorporate spatial and techno-economic data for the renewable bioenergy and biofuels supply chain network optimization. The model economically optimizes the geographic distribution and scale of biomass resources, conversion refineries, and the major fuel consumers in the United States for a 20 years’ life span.